Journal: Cell

Article Title: The cytoplasmic DNA sensor cGAS promotes mitotic cell death

doi: 10.1016/j.cell.2019.05.035

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Gassmann N/A MDA-MB-157 K. Birsoy RRID: CVCL_0618 MDA-MB-231 K. Birsoy RRID: CVCL_0062 RUES2 A. Brivanlou human ESC registry no. 0013; RRID: CVCL_VM29 T47D K. Birsoy RRID: CVCL_0553 Experimental Models: Organisms/Strains Immunodeficient mice, NOD- scid IL2Rg null (NSG), male Jackson Laboratory Cat# 005557 Xenopus laevis frogs, female NASCO Cat# LM00535M Oligonucleotides cGAS siRNA ON-TARGET plus SMARTpool Dharmacon Cat# L-015607-02 Non-targeting control siRNA Dharmacon Cat# D-001810-01 sgRNAs, see Table S2 This paper N/A QPCR primers, see Table S2 ( Wu et al., 2013 ; Wu et al., 2018 ; Yoney et al., 2018 ) N/A Recombinant DNA pcDNA5/FRT/TO ThermoFisher Cat# V652020 pX330-U6-Chimeric_BB-CBh-hSpCas9 ( Cong et al., 2013 ) Addgene Cat# 42230 cGAS bacterial expression plasmids This paper N/A Software and Algorithms CellCognition (v. 1.5.2) ( Held et al., 2010 ) http://www.cellcognition-project.org/ Graphpad Prism (v.5.0a) GraphPad Software https://www.graphpad.com/ ImageJ (v. 2.0.0-rc-43/1.51k) NIH https://imagej.nih.gov/ij/index.html SoftWoRx Applied Precision N/A Vision4D Arivis https://www.arivis.com/en/imaging-science/arivis-vision4d Open in a separate window KEY RESOURCES TABLE Nucleosomes suppress DNA-induced cGAMP synthesis by cGAS During mitotic arrest, cGAS promotes a slow buildup of IRF3 phosphorylation Phospho-IRF3 promotes mitotic apoptosis independently of transcription induction Xenograft experiments and patient data indicate a role for cGAS in taxol chemotherapy

Techniques: Recombinant, SYBR Green Assay, Expressing, Software